(I'll answer this as best I can, feel free to correct me where I'm wrong.)
In the other thread you kindly provided me with some
advice and links,
including mentioning the MIDI Tuning Standard and OSC.
This seems like a very hard problem. Since your sequencer is "just
that: a sequencer", you'll also need plugin hosts and/or standalone
synths too, which understand either MIDI tuning or OSC. (One
OSC-driven plugin host, Om, has been mentioned already). The majority
of standalone synths and plugins will probably never be able to play
anything other than a conventional tuning, so that's a problem, unless
you're happy to write a few of your own.
(By the way, re VST, some existing synths and/or plugin hosts can host
VST, but again most VSTs expect MIDI or MIDI-style commands and
implicitly assume a conventional tuning.)
I'd love to see a general-purpose OSC sequencer, and I'm sure others
would too, so keep me informed if you're thinking of taking this
approach, and maybe I can help out in some way.